Separação da proteína albumina de soro bovino utilizando hidroxiapatita e carvão ativo de babaçu (Orbignya martiana)

Abstract

Currently there has been an increased interest in the separation process of the individual whey proteins from different adsorbents, this is due to its universal applicability. The need to use adsorbents with lower costs in industrial production has been important for the separation process. The bovine serum albumin (BSA) is a protein present in bovine milk whey and has been studied as a function of their functional and nutritional qualities for biotechnological utilization purposes. This work was developed to study the process of separation of this protein at different pH (4.0 to 7.0), concentrations (0.0 to 7.0 mg g-1) and NaCl concentration in the adsorptive process. For separation were used adsorption method, using commercial hydroxyapatite (HA), synthetic hydroxyapatite (SAH) and activated carbon babassu (CAB). Initially it was performed kinetic studies of Pseudo 1st order models and Pseudo 2nd order. adsorptive efficiency of the process was also carried out. The characterization was done by analysis of the zeta potential of the adsorbents studied. For isotherms, equilibrium data were performed studies using Langmuir, Freundlich and Jovanovic. The effect of NaCl concentration was evaluated without the use of salt and (1.0 mol L-1) to the Langmuir model. The kinetic results were better represented in the Pseudo 2nd order model with chemisorption characteristics. The efficiency was improved at pH 4.0 for HA with (74.3%), with hypertension (68.7%) and CAB are obtained (37.3%). The fillers obtained from zeta potential ranging from (-6.6 and -42,8mV). The results for pH 4.0 were better, and the maximum adsorption capacity (qm): qm = 85.53 mg g-1 g-1 68.96 mg and 36.18 mg g-1 for HA, HAS and CAB respectively. For the three adsorbents noted that (qm) BSA decreased with increasing NaCl concentration. For the pH (4,0; 6,0 and 7,0), It was observed that the (qm) decreased as the pH increased indicating that electrostatic bonds and functional groups on the surface of adsorbents contributed to this reduction, particularly for the CAB, for a statistically significant difference was found (p<0.05).