Análises morfoanatômicas e fisiológicas em plantas de interesse socioambiental de Copaifera langsdorfii (Fabaceae), expostas ao glifosato

Abstract

The environmental problems arising from the intensive use of pesticides are worrying. Copaifera langsdorfii (Fabaceae), is a species native to the cerrado and inserted in the context of traditional communities that interact, from an environmental, medicina l, economic and social point of view, with this species. Therefore, the objective of this work was to verify visual, physiological and anatomical responses of C. langsdorfii plants exposed to glyphosate. An experiment was carried out with 4 treatments, 7 replications and 3 different doses of the pesticide Glyphosate (Roundup Original DI® with doses: 200, 400 and 800 g a.i. ha-1, and the control (0). The plants were exposed to a single time and kept in a greenhouse for 30 days. Visual analyzes were carried out throughout the experiment with an emphasis on phytotoxic ity. Physiological analyzes were carried out using an infrared gas analyzer (IRGA) and quantification of shikimic acid. Anatomical characterization followed paraffin inclus io n protocol and histochemical analyzes for starch and phenolic compounds. The plants showed little visual damage to their leaves, 30 days after exposure to the pesticide glyphosate. Of the physiological parameters, gas exchange was little altered and there was no change in the quantification of shikimic acid However, anatomical changes (microscopic) and, in the histochemical analysis of the cellular structures of the leaflets analyzed, were observed. These results indicate that C. langsdorfii, despite the plants showing characteristics of tolerance to glyphosate in visual and physiological parameters, suffered damage to cellular structures.